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Include cDNA conversion.For regular RNA samples, the IDT xGen RNA Library Prep Kit offers a fast NGS transcriptomic research workflow. RNA-seq libraries can be produced using tissue samples, blood, or high-quality RNA samples with an RNA Integrity Number (RIN) of 7–10. This RNA-seq workflow also utilizes Adaptase™ technology to produce libraries following first-strand cDNA synthesis (see the xGen RNA Library workflow). This workflow can be easily combined with xGen UDI Primer Plates for indexing. The xGen Broad‑Range RNA Library Prep Kit supports a wide input range, including low-quality formalin-fixed paraffin-embedded (FFPE) RNA samples. This stranded RNA-seq workflow for low-quality and/or low-input RNA samples utilizes Adaptase technology to produce libraries following first-strand cDNA synthesis (refer to xGen Broad-Range RNA Library workflow). Samples are recommended to have a RIN >2 or DV200 >30 (DV200 is a quality metric that represents the percentage of RNA fragments longer than 200 nucleotides). This workflow can also be easily combined with xGen UDI Primer Plates for indexing.After library prep, hybridization capture with IDT xGen Hyb Probes, which are individually synthesized, 5’-biotinylated oligos, enriches for fragments corresponding to the targets of interest. For researchers interested in the mRNA subset expressed from exon regions of the genome, you can pair the xGen Broad‑Range RNA Library Prep Kit or the xGen RNA Library Prep Kit with the xGen Exome Hyb Panel v2 for more efficient expression profiling, yielding a higher percentage of coding bases and a lower percentage of intronic bases than whole transcriptome data (see Figure 1B).For researchers

On the GUI on Windows. Workaround :1) Initialize the Maya environment within mayapy. import maya.standalone maya.standalone.initialize() Now os.path.expanduser() will return the same value in mayapy as itdoes in Maya. 2) Set the HOME environment variable before starting up Maya. Whateveryou set it to will be used by by os.path.expanduser() in both Maya andmayapy. UI Architecture MAYA-7804 The Ctrl-W hotkey cannot be assigned toMaya functions. It is reserved. UI General MAYA-1688 Auto-complete python commands in thescript editor will run the codes inside a Python property block, notjust returning the function/property list, but actually running theproperty itself. The same is true when you hit return to accept andfill that function/property in the script editor. XGen MAYA-23962 XGen is not compatible with merged meshes MAYA-28065 XGen: Clumping preview refresh issueWhen having a bad preview after altering clump maps, press previewagain. MAYA-35042 Loading Xgen may crash Maya iffabricengine plugin is installed.Workaround : Rename the PySide folder under the fabricengine installdirectory. MAYA-36215 Xgen Tube Groom requires tubes to havetransformations frozen MAYA-37306 Do not use spaces for your XGen project'sname on Mac. MAYA-37969 Xgen and the geo that it is bound to haveto be in the same namespace. It is not supported to import an XGendescription or collection on a piece of geo in a different namespace. MAYA-39333 Xgen control guides Soft-select falloffcolour is not yet supported. Copyright ©2014 Autodesk, Inc.

Pillage the VillageThe Sandbox Challenge The Sandbox Challenge Sandbox mode was added to Pillage the Village to allow you to utilize your creativity and now it's time to prove just how creative you are in XGen's Official "Sandbox Challenge" competition! Here's how it works: Unlock the 'Sandbox Mode' option on the main menu by reaching Level 5 and saving your game. Create the most entertaining and innovative video of you or your friends performing stunts, tricks, glitches, and scenes in Pillage the Village's sandbox mode. Capture the action using a video-capture program, such as HyperCam, and upload your video to YouTube. Each submitted video will be judged by members of the XGenStudios team, and First, Second, and Third place winners will receive prizes. Here's a sample video of Sandbox Mayhem created by the XGen Team Let the Sandbox Challenge begin! Prizes First Place- A Nintendo Wii console, customized by XGen to include hand-drawn artwork of the Rocketeer from Pillage The Village! Second Place- A customized Nintendo DS handheld gaming system, including hand-drawn characters from Pillage The Village! Third Place- Framed concept art from the early development of Pillage The Village, signed by the XGen team! How to Record & Post Video For capturing video in Sandbox Mode, we recommend using HyperCam. HyperCam is simple to use, and is a free Shareware download which you may use and evaluate for as long as you like. Download Hypercam Video and Sound recording can be very demanding tasks for a PC, especially with