Oligo analyzer

Author: m | 2025-04-24

Oligo Analyzer version 1.0 (Analyze.exe) free download, latest version 1.0.3, Oligo Analyzer is a simple tool to determine primer properties like Tm, GC% Categories Windows. Log in / Sign up. Windows › Education › Science › Oligo Analyzer › 1.0. Oligo Analyzer 1.0. Trusted Download. Free 763 KB Oligo Analyzer 1.0 Free Oligo

The Role of Oligo Analyzer and Oligo CDMO in Modern

Free Downloads: Pcr Editor For The Roland Vs License: All 1 2 | Free High performance MPEG Transport Stream Player, Recorder and Analyser supporting all Alitronika’s devices. High performance MPEG Transport Stream Player, Recorder and Analyser supporting all Alitronika’s devices. Automatically obtains the bitrate from the Pcr during Recording and Playing. Accurate estimation of bitrate when there is none in the Pcr.Unlimited numbers of play loops. Supports sub-loops, partial file play. Recoding file selectable by size by duration of recording. Category: Multimedia & Design / Multimedia App'sPublisher: Alitronika, License: Freeware, Price: USD $0.00, File Size: 26.3 MBPlatform: Windows Alitronika's DVSStation is an integrated Transport Stream Player, Recorder, Analyzer and Editor. Alitronika's DVSStation is an integrated Transport Stream Player, Recorder, Analyzer and Editor. Indeed DVSStation is the only application software one may need to make a complete DVB transport stream station, be it Transport Stream generation, recording or analyzing. DVSStation offers all the functions that one may need and what is more this powerful software is FREE with every... Category: Audio / Utilities & Plug-InsPublisher: Alitronika, License: Demo, Price: USD $0.00, File Size: 26.3 MBPlatform: Windows PrimersList analyzes different features of multiple primers simultaneously, the melting temperature calculation for standard and degenerate oligonucleotides, GC content, primer PCR efficiency. PrimersList analyzes different features of multiple primers simultaneously, the melting temperature calculation for standard and degenerate oligonucleotides, GC content, primer Pcr efficiency. Primers are analyzed for all primer secondary structures including G-quadruplexes detection, hairpins, self-dimers and cross-dimers in primer pairs; sequence linguistic complexity, molecular... Category: Business & Finance / ApplicationsPublisher: Ruslan Kalendar, License: Freeware, Price: USD $0.00, File Size: 153 BPlatform: Windows Oligo Analyzer is a powerful and easy-to-use tool to evaluate physical properties of primers. Oligo Analyzer is a powerful and easy-to-use tool to evaluate physical properties of primers.Supports 'Oligo Analyzer project'

Oligo Analyzer 1.0.3 - Download, Review

Page you will see a simple map showing the assembly so far. Note that the insert 5' end is joined to base 100 and the 3' end is joined to base 151 in the vector. Let's add another fragment.Click new fragment. From the dropdown, we are going to select GFP as our third fragment. Name the fragment. Select PCR as the method of production. Check the start and end bases. In this case, we will be adding the entire sequence. Click add. On the next page, you will see that the map on the right now shows the construct containing the three selected fragments depicted in three different colors. The fragments can be rearranged by dragging and dropping the colored bars into the preferred order. You can also delete fragments or edit them by using the functionalities/icons shown in the colored bars. You can also click the bars to view the assembly junctions, primer sequences and the amino acid sequence for all six reading frames. The notes section provides information about the characteristics of the primers. The build settings shows the default settings used to generate the primers.Use this to change the assembly product, minimum overlap size or polymerase used for PCR. Now click done. On the next page there will be a summary of your assembly. The summary contains a simple map of the assembled product, a sequence and multiple formats that can be exported and a color-coded sequence so that you can easily identify the assembly junctions. The required oligo section shows the oligo sequence which can be shown in FASTA or IDT compatible sequence formats as well as the Tm and annealing temperature of those oligos. The lower case five prime end is the overlap region of the primer. It is color-coded to match the color of the adjacent fragment in the assembly. The upper case 3' end is the gene specific portion of the oligo that acts as the primer for PCR. It is also color coded to match the PCR template. Use the load/save tab to save your assembly. Enter the name of the assembly in the save project field and export the file to your computer. To start a new assembly, click build and then start over. Click okay to delete the existing project and then you can start your next assembly.

Oligo Analyzer from IDT - YouTube

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Analyze: Hairpin Stems Windows - Oligo

PrimerAnalyserprovides comprehensive analysis of sequence with standard and mixed bases, as well as DNA/RNA, methylated, locked and phosphorothioated bases; tool will calculate the physical properties of the sequence including length, GC% content, melting temperature, molecular weight, the extiction coefficient, the optical density (OD), sequence linguistic complexity, self-dimers and G-quadruplexes detection.A name is not required for oligonucleotide (eg. agtcagtcagtcagtcagtc).Degenerate primer sequences are also accepted, each letter represents a combination of one or several nucleotides: B=(C,G,T), D=(A,G,T), H=(A,C,T), K=(G,T), M=(A,C), N=(A,C,G,T), R=(A,G), S=(G,C), V=(A,C,G), W=(A,T), Y=(C,T). U=Uracil, I=Inosine and LNA: dA=E, dC=F, dG=J, dT=L. Linguistic sequence complexity (LC%) is a measure of the 'vocabulary richness' of a genetic text based on counting the number of possible combinations of nucleotides ("entropy" of the set of possibilities) to the theoretically possible one. This value for sequence is converted to percentages, 100% being the highest level. Melting temperature calculations are based on nearest neighbor thermodynamic parameters for standard and degenerate oligonucleotides, including LNA and other modifications. And provides a dilution and resuspension calculator for stocks.The Analyzer provides the following results: Tm (°C)CG content (%)Sequence linguistic complexity (LC%)Length of the primers (nt)Number of individual bases (A, T, C and G)Extinction coefficient (l/(mol·cm))Molecular weight (g/mol)Amount/OD unit (nmol/OD260)Mass (µg/OD260)Primer-dimer estimation Type or paste sequence of oligo with universal degenerate DNA code (5'-3'). Results appear instantly in the output fields (lower windows) and update automatically as you make changes to the sequences. Parameters for calculation of oligonucleotide Tm:Dilution of oligo:Primer concentration (0.01-5 µM):Target volume (µl):Total salt (Na+, K+, NH4+, Tris+) concentration (1-1000 mM):Target concentration (µM):Mg2+ concentration (0-10 mM):Volume of the stock (µl):Value of the sensivity for primer dimer detection (4-10):MQ water or TE buffer (µl):Amount of oligo:Preparing stock solution:OD (A260):Stock concentration (µM):Mass (µg):nmol:To export the results: select all (Ctrl-A), copy (Ctrl-C) and paste (Ctrl-V).

Analyze: Tm analysis window - Oligo

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Analyze: Selected primer sets - Oligo

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